Chromatography

Chromatography is essentially a physical method of separation in which the components of a mixture are separated by their distribution between two phases; one of these phases in the form of a porous bed, bulk liquid, layer or film is generally immobile (stationary phase), while the other is a fluid (mobile phase) that percolates through or over the stationary phase.

 

Individual compounds are distinguished by their ability to participate in common intermolecular interactions in the two phases, which can generally be characterized by an equilibrium constant, and is thus a property predicted from chemical thermodynamics. Interactions are mainly physical in type or involve weak chemical bonds, for example dipole–dipole, hydrogen bond formation, charge transfer, and reversible, since useful separations only result if the compound spends some time in both phases.

Chromatography_edited.jpg

Our services:

  • Recombinant Protein Purification (IMAC, GST)
     

  • Molecular Exclusion Chromatography
     

  • Ion Exchange Chromatography (Cationic/Anionic)
     

  • Antibody Purification
     

  • Affinity Chromatography
     

  • Customized service

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