Bottom-up proteomics (DDA - Massive Method)
Updated: Dec 23, 2021
Our Core Facility of Proteomics & Genomics has a platform integrated by the areas of proteomics, genomics, chromatography and bioinformatics. Each of these platforms is made up of the best and most robust technology, highly trained researchers, as well as a diverse variety of mounted techniques that ensure the highest standard in each of our services.
Next, we will tell you about one of our workflows: Botton-up proteomics (DDA - Massive Method).
Data processing begins with complex samples of diverse nature, such as soil, bacteria, animal, plant, among other origins, which are prepared for subsequent steps. These samples can be entered directly into our timsTOF Pro mass spectrometer obtaining a single run or, conversely, entered into our ÄKTA Avant 25 chromatograph.
Through this step, the initial samples are decomplexed (a process called "Deep proteomics"), obtaining 8 independent and deeper fractions that will then enter the mass spectrometry platform.
Either 8 independent fractions or a simple run, later they enter the search engine composed of the PEAKS Studio X + software and a database with reference proteomes (for example, humans, flies, yeasts, among others), with this We will identify the proteins, their intensity, their spectral count, and the number of peptides present in the initial samples.
To learn more about each of our services, visit our page: melisainstitute.org/core-facility