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Button-up proteomics (DIA-Massive Method)

Updated: Dec 23, 2021

Flujo de Trabajo Button-up proteomics, MELISA Institute

Our Proteomics and Genomics Core Facility has a platform integrated by the areas of proteomics, genomics, chromatography and bioinformatics. Each of these platforms is composed of the best and most robust technology, highly trained researchers, as well as a diverse variety of mounted techniques that ensure the highest standard in each of our services.

Next, we tell you about one of our workflows: Button-up proteomics (DIA-Massive Method) The Data Independent Acquisition (DIA) method requires two steps.

First, it is necessary to create a spectral library by means of the Data Dependent Acquisition (DDA) method that will be used later. Based on this, we will have as many spectra as possible within a specific sample.

Secondly, it is necessary to perform a run of LC-MS/MS in dia-PASEF mode. Broadly speaking, it shares a methodology of protein extraction and digestion similar to that of DDA, having variations in proteomic data capture and bioinformatics analysis.

From our previously created spectral library, we will identify the proteins contained in the DIA results, in addition to their intensity, spectral count and number of peptides present in the initial samples, obtaining the final result.

One of the great advantages of our timsTOF Pro proteomics platform and our dia-PASEF-based workflow is its performance in complex projects that require a high number of mass spectrometry runs, because the DIA technique is especially beneficial for the massive identification of proteins in samples of various nature.

To learn more about each of our services, visit our page, section Core Facility.

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