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  • Writer's pictureMelisa Institute

MELISA’s Experts: Our Core Facility in the words of its manager, Cristian Vargas, MD.

Updated: Dec 23, 2021

MELISA Institute

MELISA Institute's Core Facility is a services division aimed at providing personalized solutions to researchers and companies in the field of massive protein and nucleic acid analysis technologies through omics sciences such as genomics, transcriptomics, proteomics and bioinformatics.

Currently, our services for researchers are oriented in these 4 areas (Proteomics, Genomics, Chromatography and Bioinformatics) and 7 areas of Research and Development for the generation of new technologies applied to industry in areas such as life sciences, agriculture, food, forestry, biomedicine, microbiology and mining.

According to our CEO and Core Facility Manager, Cristian Vargas, MD; research services in our proteomics area are based on the bottom-up proteomics method and its applications such as Label-Free Quantification (LFQ), Tandem Mass Tagging (TMT), Sequential Window Acquisition of all Theorical Fragment ion spectra Mass Spectrometry (SWATH MS), Selected Reaction Monitoring (SRM), de novo peptidomics, analysis of post-translational modifications, metaproteomics, antibody sequencing, among others.

In the area of ​​genomics, our research services are RNA-Seq, whole-genome sequencing, de novo genome sequencing, targeted sequencing of genomic regions of interest, exome sequencing and analysis, real-time PCR for analyzing gene expression, metagenomics analyzes, genotyping / targeted genotyping, microbiological analyzes.

Our research services in chromatography are purification of recombinant proteins (IMAC, GST), molecular exclusion chromatography, ion exchange chromatography (cationic and anionic) and purification of antibodies.

In the bioinformatics area, our services include annotation and functional enrichment of protein sequences, quantitative analysis of proteins under different methodologies, pathway analysis, analysis of post-translational modifications, and analysis of protein and peptide sequences.

Closing the list, in the field of research and development, we offer comprehensive solutions tailored to the requirements of the agricultural, food, forestry, biomedical, microbiology and mining industries, through technology contracts.

First-class instruments for research

Our proteomics platform is composed of a Bruker Daltonics's tims TOF Pro mass spectrometer coupled to a NanoELUTE nanochromatograph, a General Electric's AKTA Avant 25 chromatograph, a Direct Detect infrared spectrometer for peptide-protein quantification and a HyperVAC-Max-VC2200 HyperCool freeze dryer with concentrator rotary.

The genomics platform is comprised of an Illumina's NEXTSeq 500 massive sequencer, an Applied Biosystem's Step One Plus thermal cycler, a Thermo Fisher Scientific's Qubit fluorometer, and a Qsep100 BiOptics capillary electrophoresis system.

The bioinformatics platform is made up of an analysis server consisting of a dual Intel Xeon E5-2650 v4 processor with 24 processing threads each (48 threads), 512 Gb of RAM and 50 Tb of storage. In addition, we have Peaks Studio X + software from Bioinformatics Solutions Inc. and software specialized in proteomics such as Skyline, MSFragger, MaxQuant, Perseus among others.

The equipment of our Core Facility, together with the dedication of our laboratory staff, allows MELISA Institute to offer first-rate service experiences for companies and researchers.

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